rg 108 Search Results


rg108  (Tocris)
92
Tocris rg108
a, Heat map of RNA-seq data showing levels of the differentially regulated SAM-utilizing enzymes. Plotted data are expressed as mean centered values. b, Expression of DNMT1 and DNMT3A in K, KL, and KL cells transduced with LKB1 cDNA (rescue) was measured by qRT-PCR. Levels were normalized to 18S rRNA. Data are expressed as relative to K cells (n=4, representative of two experiments). c, Immunoblots of lysates from K, KL or ‘rescue’ cells were probed for DNMT1 or DNMT3A. Actin is used as loading control. For gel source see Supplementary Data Figure 1. d, Measurement of SAM in K and KL cells treated with 5-Aza-2-deoxycytidine (Decitabine) or <t>RG108</t> for 3 days. In each case, data are expressed as relative to the amount of SAM in K-vehicle treated cells that is arbitrarily set to 1 (n=6 independent replicates). e, Immunofluorescence staining and quantitation of 5-methyl-cytosine (5mC) in K or KL cells (77–130 cells). Scale bar, 25 μm. f, Dot blot of DNA isolated from K or KL cells probed with anti-5-methyl-cytosine antibody (5mC). Quantitated signal was normalized to total DNA as measured by methylene blue staining (n=4, independent replicates). g, Dot blot of DNA isolated from KL cells transduced with empty vector or LKB1 cDNA probed with anti-5mC antibody. Quantitated signal was normalized to total DNA as measured by methylene blue staining (n=3, independent replicates). h, Immunoblot analysis of histone 3 (H3) methyl marks from K or KL cells. Data are normalized to total H3 (K4me3, n=2, K27me3, n=5, K36me3, n=5, independent replicates). For gel source see Supplementary Data Figure 1. i, Dot blot of DNA isolated from K or KL cells probed with anti-5-hydroxymethyl-cytosine antibody (5hmC). Quantitated signal is normalized to total DNA as measured by methylene blue staining (n=4, independent replicates). For all panels, error bars are standard deviation unless otherwise stated and statistical significance is indicated as follows: *P<0.05, **P<0.01, ***P<0.001.
Rg108, supplied by Tocris, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rg108/product/Tocris
Average 92 stars, based on 1 article reviews
rg108 - by Bioz Stars, 2026-03
92/100 stars
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dna methyltransferase inhibitor  (Santa Cruz Biotechnology)
92
Santa Cruz Biotechnology dna methyltransferase inhibitor
a, Heat map of RNA-seq data showing levels of the differentially regulated SAM-utilizing enzymes. Plotted data are expressed as mean centered values. b, Expression of DNMT1 and DNMT3A in K, KL, and KL cells transduced with LKB1 cDNA (rescue) was measured by qRT-PCR. Levels were normalized to 18S rRNA. Data are expressed as relative to K cells (n=4, representative of two experiments). c, Immunoblots of lysates from K, KL or ‘rescue’ cells were probed for DNMT1 or DNMT3A. Actin is used as loading control. For gel source see Supplementary Data Figure 1. d, Measurement of SAM in K and KL cells treated with 5-Aza-2-deoxycytidine (Decitabine) or <t>RG108</t> for 3 days. In each case, data are expressed as relative to the amount of SAM in K-vehicle treated cells that is arbitrarily set to 1 (n=6 independent replicates). e, Immunofluorescence staining and quantitation of 5-methyl-cytosine (5mC) in K or KL cells (77–130 cells). Scale bar, 25 μm. f, Dot blot of DNA isolated from K or KL cells probed with anti-5-methyl-cytosine antibody (5mC). Quantitated signal was normalized to total DNA as measured by methylene blue staining (n=4, independent replicates). g, Dot blot of DNA isolated from KL cells transduced with empty vector or LKB1 cDNA probed with anti-5mC antibody. Quantitated signal was normalized to total DNA as measured by methylene blue staining (n=3, independent replicates). h, Immunoblot analysis of histone 3 (H3) methyl marks from K or KL cells. Data are normalized to total H3 (K4me3, n=2, K27me3, n=5, K36me3, n=5, independent replicates). For gel source see Supplementary Data Figure 1. i, Dot blot of DNA isolated from K or KL cells probed with anti-5-hydroxymethyl-cytosine antibody (5hmC). Quantitated signal is normalized to total DNA as measured by methylene blue staining (n=4, independent replicates). For all panels, error bars are standard deviation unless otherwise stated and statistical significance is indicated as follows: *P<0.05, **P<0.01, ***P<0.001.
Dna Methyltransferase Inhibitor, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dna methyltransferase inhibitor/product/Santa Cruz Biotechnology
Average 92 stars, based on 1 article reviews
dna methyltransferase inhibitor - by Bioz Stars, 2026-03
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rg-108  (Active Motif)
90
Active Motif rg-108
a, Heat map of RNA-seq data showing levels of the differentially regulated SAM-utilizing enzymes. Plotted data are expressed as mean centered values. b, Expression of DNMT1 and DNMT3A in K, KL, and KL cells transduced with LKB1 cDNA (rescue) was measured by qRT-PCR. Levels were normalized to 18S rRNA. Data are expressed as relative to K cells (n=4, representative of two experiments). c, Immunoblots of lysates from K, KL or ‘rescue’ cells were probed for DNMT1 or DNMT3A. Actin is used as loading control. For gel source see Supplementary Data Figure 1. d, Measurement of SAM in K and KL cells treated with 5-Aza-2-deoxycytidine (Decitabine) or <t>RG108</t> for 3 days. In each case, data are expressed as relative to the amount of SAM in K-vehicle treated cells that is arbitrarily set to 1 (n=6 independent replicates). e, Immunofluorescence staining and quantitation of 5-methyl-cytosine (5mC) in K or KL cells (77–130 cells). Scale bar, 25 μm. f, Dot blot of DNA isolated from K or KL cells probed with anti-5-methyl-cytosine antibody (5mC). Quantitated signal was normalized to total DNA as measured by methylene blue staining (n=4, independent replicates). g, Dot blot of DNA isolated from KL cells transduced with empty vector or LKB1 cDNA probed with anti-5mC antibody. Quantitated signal was normalized to total DNA as measured by methylene blue staining (n=3, independent replicates). h, Immunoblot analysis of histone 3 (H3) methyl marks from K or KL cells. Data are normalized to total H3 (K4me3, n=2, K27me3, n=5, K36me3, n=5, independent replicates). For gel source see Supplementary Data Figure 1. i, Dot blot of DNA isolated from K or KL cells probed with anti-5-hydroxymethyl-cytosine antibody (5hmC). Quantitated signal is normalized to total DNA as measured by methylene blue staining (n=4, independent replicates). For all panels, error bars are standard deviation unless otherwise stated and statistical significance is indicated as follows: *P<0.05, **P<0.01, ***P<0.001.
Rg 108, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rg-108/product/Active Motif
Average 90 stars, based on 1 article reviews
rg-108 - by Bioz Stars, 2026-03
90/100 stars
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0.04 μm rg-108  (Merck & Co)
90
Merck & Co 0.04 μm rg-108
a, Heat map of RNA-seq data showing levels of the differentially regulated SAM-utilizing enzymes. Plotted data are expressed as mean centered values. b, Expression of DNMT1 and DNMT3A in K, KL, and KL cells transduced with LKB1 cDNA (rescue) was measured by qRT-PCR. Levels were normalized to 18S rRNA. Data are expressed as relative to K cells (n=4, representative of two experiments). c, Immunoblots of lysates from K, KL or ‘rescue’ cells were probed for DNMT1 or DNMT3A. Actin is used as loading control. For gel source see Supplementary Data Figure 1. d, Measurement of SAM in K and KL cells treated with 5-Aza-2-deoxycytidine (Decitabine) or <t>RG108</t> for 3 days. In each case, data are expressed as relative to the amount of SAM in K-vehicle treated cells that is arbitrarily set to 1 (n=6 independent replicates). e, Immunofluorescence staining and quantitation of 5-methyl-cytosine (5mC) in K or KL cells (77–130 cells). Scale bar, 25 μm. f, Dot blot of DNA isolated from K or KL cells probed with anti-5-methyl-cytosine antibody (5mC). Quantitated signal was normalized to total DNA as measured by methylene blue staining (n=4, independent replicates). g, Dot blot of DNA isolated from KL cells transduced with empty vector or LKB1 cDNA probed with anti-5mC antibody. Quantitated signal was normalized to total DNA as measured by methylene blue staining (n=3, independent replicates). h, Immunoblot analysis of histone 3 (H3) methyl marks from K or KL cells. Data are normalized to total H3 (K4me3, n=2, K27me3, n=5, K36me3, n=5, independent replicates). For gel source see Supplementary Data Figure 1. i, Dot blot of DNA isolated from K or KL cells probed with anti-5-hydroxymethyl-cytosine antibody (5hmC). Quantitated signal is normalized to total DNA as measured by methylene blue staining (n=4, independent replicates). For all panels, error bars are standard deviation unless otherwise stated and statistical significance is indicated as follows: *P<0.05, **P<0.01, ***P<0.001.
0.04 μm Rg 108, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/0.04 μm rg-108/product/Merck & Co
Average 90 stars, based on 1 article reviews
0.04 μm rg-108 - by Bioz Stars, 2026-03
90/100 stars
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rg-108  (Federation of European Neuroscience Societies)
90
Federation of European Neuroscience Societies rg-108
a, Heat map of RNA-seq data showing levels of the differentially regulated SAM-utilizing enzymes. Plotted data are expressed as mean centered values. b, Expression of DNMT1 and DNMT3A in K, KL, and KL cells transduced with LKB1 cDNA (rescue) was measured by qRT-PCR. Levels were normalized to 18S rRNA. Data are expressed as relative to K cells (n=4, representative of two experiments). c, Immunoblots of lysates from K, KL or ‘rescue’ cells were probed for DNMT1 or DNMT3A. Actin is used as loading control. For gel source see Supplementary Data Figure 1. d, Measurement of SAM in K and KL cells treated with 5-Aza-2-deoxycytidine (Decitabine) or <t>RG108</t> for 3 days. In each case, data are expressed as relative to the amount of SAM in K-vehicle treated cells that is arbitrarily set to 1 (n=6 independent replicates). e, Immunofluorescence staining and quantitation of 5-methyl-cytosine (5mC) in K or KL cells (77–130 cells). Scale bar, 25 μm. f, Dot blot of DNA isolated from K or KL cells probed with anti-5-methyl-cytosine antibody (5mC). Quantitated signal was normalized to total DNA as measured by methylene blue staining (n=4, independent replicates). g, Dot blot of DNA isolated from KL cells transduced with empty vector or LKB1 cDNA probed with anti-5mC antibody. Quantitated signal was normalized to total DNA as measured by methylene blue staining (n=3, independent replicates). h, Immunoblot analysis of histone 3 (H3) methyl marks from K or KL cells. Data are normalized to total H3 (K4me3, n=2, K27me3, n=5, K36me3, n=5, independent replicates). For gel source see Supplementary Data Figure 1. i, Dot blot of DNA isolated from K or KL cells probed with anti-5-hydroxymethyl-cytosine antibody (5hmC). Quantitated signal is normalized to total DNA as measured by methylene blue staining (n=4, independent replicates). For all panels, error bars are standard deviation unless otherwise stated and statistical significance is indicated as follows: *P<0.05, **P<0.01, ***P<0.001.
Rg 108, supplied by Federation of European Neuroscience Societies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rg-108/product/Federation of European Neuroscience Societies
Average 90 stars, based on 1 article reviews
rg-108 - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


a, Heat map of RNA-seq data showing levels of the differentially regulated SAM-utilizing enzymes. Plotted data are expressed as mean centered values. b, Expression of DNMT1 and DNMT3A in K, KL, and KL cells transduced with LKB1 cDNA (rescue) was measured by qRT-PCR. Levels were normalized to 18S rRNA. Data are expressed as relative to K cells (n=4, representative of two experiments). c, Immunoblots of lysates from K, KL or ‘rescue’ cells were probed for DNMT1 or DNMT3A. Actin is used as loading control. For gel source see Supplementary Data Figure 1. d, Measurement of SAM in K and KL cells treated with 5-Aza-2-deoxycytidine (Decitabine) or RG108 for 3 days. In each case, data are expressed as relative to the amount of SAM in K-vehicle treated cells that is arbitrarily set to 1 (n=6 independent replicates). e, Immunofluorescence staining and quantitation of 5-methyl-cytosine (5mC) in K or KL cells (77–130 cells). Scale bar, 25 μm. f, Dot blot of DNA isolated from K or KL cells probed with anti-5-methyl-cytosine antibody (5mC). Quantitated signal was normalized to total DNA as measured by methylene blue staining (n=4, independent replicates). g, Dot blot of DNA isolated from KL cells transduced with empty vector or LKB1 cDNA probed with anti-5mC antibody. Quantitated signal was normalized to total DNA as measured by methylene blue staining (n=3, independent replicates). h, Immunoblot analysis of histone 3 (H3) methyl marks from K or KL cells. Data are normalized to total H3 (K4me3, n=2, K27me3, n=5, K36me3, n=5, independent replicates). For gel source see Supplementary Data Figure 1. i, Dot blot of DNA isolated from K or KL cells probed with anti-5-hydroxymethyl-cytosine antibody (5hmC). Quantitated signal is normalized to total DNA as measured by methylene blue staining (n=4, independent replicates). For all panels, error bars are standard deviation unless otherwise stated and statistical significance is indicated as follows: *P<0.05, **P<0.01, ***P<0.001.

Journal: Nature

Article Title: LKB1 loss links serine metabolism to DNA methylation and tumourigenesis

doi: 10.1038/nature20132

Figure Lengend Snippet: a, Heat map of RNA-seq data showing levels of the differentially regulated SAM-utilizing enzymes. Plotted data are expressed as mean centered values. b, Expression of DNMT1 and DNMT3A in K, KL, and KL cells transduced with LKB1 cDNA (rescue) was measured by qRT-PCR. Levels were normalized to 18S rRNA. Data are expressed as relative to K cells (n=4, representative of two experiments). c, Immunoblots of lysates from K, KL or ‘rescue’ cells were probed for DNMT1 or DNMT3A. Actin is used as loading control. For gel source see Supplementary Data Figure 1. d, Measurement of SAM in K and KL cells treated with 5-Aza-2-deoxycytidine (Decitabine) or RG108 for 3 days. In each case, data are expressed as relative to the amount of SAM in K-vehicle treated cells that is arbitrarily set to 1 (n=6 independent replicates). e, Immunofluorescence staining and quantitation of 5-methyl-cytosine (5mC) in K or KL cells (77–130 cells). Scale bar, 25 μm. f, Dot blot of DNA isolated from K or KL cells probed with anti-5-methyl-cytosine antibody (5mC). Quantitated signal was normalized to total DNA as measured by methylene blue staining (n=4, independent replicates). g, Dot blot of DNA isolated from KL cells transduced with empty vector or LKB1 cDNA probed with anti-5mC antibody. Quantitated signal was normalized to total DNA as measured by methylene blue staining (n=3, independent replicates). h, Immunoblot analysis of histone 3 (H3) methyl marks from K or KL cells. Data are normalized to total H3 (K4me3, n=2, K27me3, n=5, K36me3, n=5, independent replicates). For gel source see Supplementary Data Figure 1. i, Dot blot of DNA isolated from K or KL cells probed with anti-5-hydroxymethyl-cytosine antibody (5hmC). Quantitated signal is normalized to total DNA as measured by methylene blue staining (n=4, independent replicates). For all panels, error bars are standard deviation unless otherwise stated and statistical significance is indicated as follows: *P<0.05, **P<0.01, ***P<0.001.

Article Snippet: Laminin (23017-95), 2NBDG (N13195), DCFDA (C6827), CellROX Green ( C10444 ), CyQuant NF ( C35006 ), bovine pituitary extract (13038-14), HBSS, RPMI, DMEM, DMEM:F12, MEM penicillin-streptomycin, pyruvate solution, fetal bovine serum (FBS) Alexa 488 and 568-conjugated secondary antibodies from Life Technologies; nicotinamide (N3376), trypsin inhibitor (T6522), dexamethasone (D4902), S-adenosylmethionine (A2408), 3,3,5-tri-iodo-L-thyronine (91990), oligomycin (75351), antimycin A (A8674), 2-deoxyglucose (D6134), dichloroacetate (347795), collagenase (C7657), glucose (G7528), aminooxyacetate ( C13408 ), N-acetylcysteine (A9165), adenosine (A4036), guanosine (G6264), thymidine (T1895), uridine (U3003), cytidine (C4654), cycloleucine (A48105), cholera toxin (C8052), L-serine (S4500), betaine (61962), dimethylglycine (D1156), tamoxifen (T5148) and methylene blue (M4159) from Sigma; FCCP (0453), decitabine (2624), RG108 (3295), EGCG (4524), Compound C (3093), Torin 1 (4247) and galloflavin (4795) from Tocris; mouse EGF (354001), NuSerum IV (355104), ITS+ Premix (354352) and matrigel (354234) from Corning; SGI1027 (S7276) from Selleckchem; caspase-Glo 3/7 kit (G811A) and Celltiter-Glo kit (G755B) from Promega; 13 C-U-Glucose (CLM-1396-1) and 15 N-Glutamine (NLM-1016-1) from Cambridge Isotope Laboratories Inc; dispase (165-859) from Roche; lactate assay kit (K607-100) from Biovision Inc; Vectashield with DAPI (H1500) from Vector Laboratories; Bridge-IT SAM assay kit (1-1-1003B) from Medionics; meDIP kit (55009) from Active Motif; 3DZA (9000785) from Cayman Chemicals; Adeno-FlpO (1760) from Vector Biolabs; Adeno-Cre-eGFP from the Viral Vector Core Facility, University of Iowa.

Techniques: Expressing, DNA Methylation Assay, RNA Sequencing, Transduction, Quantitative RT-PCR, Western Blot, Control, Immunofluorescence, Staining, Quantitation Assay, Dot Blot, Isolation, Plasmid Preparation, Standard Deviation

a–c, Proliferation of K (a), KL (b) and KPC (c) cells transduced with shControl or two hairpins against each of DNMT1 (D1) or DNMT3A (D3A). Data are expressed as relative to day 0 (K and KL n=6, KPC n=4). d–f, Volume of subcutaneous tumours derived from KPC cells transduced with Doxcycline (Dox)-inducible hairpins against DNMT1 or DNMT3A (n=4). Dox was introduced to the drinking water when tumours reached 125 mm3. Error bars are s.e.m. For source data on tumour volume, see Supplementary Data Table 4. g, Apoptosis measured by caspase 3/7 activity in K or KL cells treated with decitabine for 48 hrs. Values are normalized to cell number (n=3). h and i, Proliferation of KL cells transduced with empty vector (h) or LKB1 cDNA (i) treated with decitabine. Data are expressed as relative to day 0 (n=3). j, Proliferation of KL cells transduced with empty vector or LKB1 cDNA treated with RG108. Data are expressed as percentage of growth of untreated cells (n=6). k–m, Proliferation of K or KL cells treated with RG108 (n=6) (k), EGCG (n=12) (l) or SGI1027 (n=12) (m). Data are expressed as percentage of growth of untreated cells. n–q, Mice bearing subcutaneous KPC tumours were treated with decitabine (n=12) or vehicle (n=12) when tumours reached 125 mm3. n and o, Tumour volume (n) and final tumour weight (o). Error bars are s.e.m. For source data on tumour volume, see Supplementary Data Table 4. p, H&E stained slides from representative tumours (upper panels). Lower panels: Anti-CK19 (green) was used to visualize the neoplastic epithelium and anti-PCNA (red) was used to mark proliferating cells. DAPI was used to stain nuclei (blue). q, Quantitation of the CK19+ neoplastic epithelial compartment (%CK19+ cells/total cells) (Upper). Quantification of CK19+ cells with nuclear PCNA staining (Lower) (n=6). Scale bars, 100 μm. Insets are three-fold magnification. Data pooled from 2 (a–c, j) or four (k–m) experiments or representative of two (h) or three (g) experiments. For all panels, error bars are standard deviation unless otherwise stated and statistical significance is indicated as follows: *P<0.05, **P<0.01, ***P<0.001.

Journal: Nature

Article Title: LKB1 loss links serine metabolism to DNA methylation and tumourigenesis

doi: 10.1038/nature20132

Figure Lengend Snippet: a–c, Proliferation of K (a), KL (b) and KPC (c) cells transduced with shControl or two hairpins against each of DNMT1 (D1) or DNMT3A (D3A). Data are expressed as relative to day 0 (K and KL n=6, KPC n=4). d–f, Volume of subcutaneous tumours derived from KPC cells transduced with Doxcycline (Dox)-inducible hairpins against DNMT1 or DNMT3A (n=4). Dox was introduced to the drinking water when tumours reached 125 mm3. Error bars are s.e.m. For source data on tumour volume, see Supplementary Data Table 4. g, Apoptosis measured by caspase 3/7 activity in K or KL cells treated with decitabine for 48 hrs. Values are normalized to cell number (n=3). h and i, Proliferation of KL cells transduced with empty vector (h) or LKB1 cDNA (i) treated with decitabine. Data are expressed as relative to day 0 (n=3). j, Proliferation of KL cells transduced with empty vector or LKB1 cDNA treated with RG108. Data are expressed as percentage of growth of untreated cells (n=6). k–m, Proliferation of K or KL cells treated with RG108 (n=6) (k), EGCG (n=12) (l) or SGI1027 (n=12) (m). Data are expressed as percentage of growth of untreated cells. n–q, Mice bearing subcutaneous KPC tumours were treated with decitabine (n=12) or vehicle (n=12) when tumours reached 125 mm3. n and o, Tumour volume (n) and final tumour weight (o). Error bars are s.e.m. For source data on tumour volume, see Supplementary Data Table 4. p, H&E stained slides from representative tumours (upper panels). Lower panels: Anti-CK19 (green) was used to visualize the neoplastic epithelium and anti-PCNA (red) was used to mark proliferating cells. DAPI was used to stain nuclei (blue). q, Quantitation of the CK19+ neoplastic epithelial compartment (%CK19+ cells/total cells) (Upper). Quantification of CK19+ cells with nuclear PCNA staining (Lower) (n=6). Scale bars, 100 μm. Insets are three-fold magnification. Data pooled from 2 (a–c, j) or four (k–m) experiments or representative of two (h) or three (g) experiments. For all panels, error bars are standard deviation unless otherwise stated and statistical significance is indicated as follows: *P<0.05, **P<0.01, ***P<0.001.

Article Snippet: Laminin (23017-95), 2NBDG (N13195), DCFDA (C6827), CellROX Green ( C10444 ), CyQuant NF ( C35006 ), bovine pituitary extract (13038-14), HBSS, RPMI, DMEM, DMEM:F12, MEM penicillin-streptomycin, pyruvate solution, fetal bovine serum (FBS) Alexa 488 and 568-conjugated secondary antibodies from Life Technologies; nicotinamide (N3376), trypsin inhibitor (T6522), dexamethasone (D4902), S-adenosylmethionine (A2408), 3,3,5-tri-iodo-L-thyronine (91990), oligomycin (75351), antimycin A (A8674), 2-deoxyglucose (D6134), dichloroacetate (347795), collagenase (C7657), glucose (G7528), aminooxyacetate ( C13408 ), N-acetylcysteine (A9165), adenosine (A4036), guanosine (G6264), thymidine (T1895), uridine (U3003), cytidine (C4654), cycloleucine (A48105), cholera toxin (C8052), L-serine (S4500), betaine (61962), dimethylglycine (D1156), tamoxifen (T5148) and methylene blue (M4159) from Sigma; FCCP (0453), decitabine (2624), RG108 (3295), EGCG (4524), Compound C (3093), Torin 1 (4247) and galloflavin (4795) from Tocris; mouse EGF (354001), NuSerum IV (355104), ITS+ Premix (354352) and matrigel (354234) from Corning; SGI1027 (S7276) from Selleckchem; caspase-Glo 3/7 kit (G811A) and Celltiter-Glo kit (G755B) from Promega; 13 C-U-Glucose (CLM-1396-1) and 15 N-Glutamine (NLM-1016-1) from Cambridge Isotope Laboratories Inc; dispase (165-859) from Roche; lactate assay kit (K607-100) from Biovision Inc; Vectashield with DAPI (H1500) from Vector Laboratories; Bridge-IT SAM assay kit (1-1-1003B) from Medionics; meDIP kit (55009) from Active Motif; 3DZA (9000785) from Cayman Chemicals; Adeno-FlpO (1760) from Vector Biolabs; Adeno-Cre-eGFP from the Viral Vector Core Facility, University of Iowa.

Techniques: DNA Methylation Assay, Transduction, Derivative Assay, Activity Assay, Plasmid Preparation, Staining, Quantitation Assay, Standard Deviation